1LEV
PORCINE KIDNEY FRUCTOSE-1,6-BISPHOSPHATASE COMPLEXED WITH AN AMP-SITE INHIBITOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-23 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.870, 73.446, 78.025 |
| Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
| Resolution | 40.260 - 2.150 |
| R-factor | 0.19582 |
| Rwork | 0.193 |
| R-free | 0.28300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fbp |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.260 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.106 | 0.483 |
| Number of reflections | 32736 | |
| <I/σ(I)> | 13 | 2.44 |
| Completeness [%] | 93.8 | 64.5 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2M Na Acetate, 13.75% PEG 4000, 0.1M Hepes pH7.0, 1mM F6P, 2mM MnCl2, 1mM inhibitor, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium phosphate | 8 (M) | pH7.2 |
| 10 | 1 | reservoir | PEG4000 | 13.5 (%) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
| 4 | 1 | drop | 2.0 (mM) | ||
| 5 | 1 | drop | compound 1 | 1 (mM) | |
| 6 | 1 | drop | protein | 15 (mg/ml) | |
| 7 | 1 | drop | fructose6-phosphate | 2 (mM) | |
| 8 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
| 9 | 1 | reservoir | sodium acetate | 200 (mM) |
