1LEI
The kB DNA sequence from the HLV-LTR functions as an allosteric regulator of HIV transcription
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 137.131, 179.697, 97.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| Rwork | 0.250 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.330 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.064 | 0.315 * |
| Total number of observations | 254045 * | |
| Number of reflections | 33470 | |
| <I/σ(I)> | 13.8 | 2.5 |
| Completeness [%] | 98.0 | 89 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 18 * | PEG 3350, CaCl2, sodium spermine, pH 6.5, SMALL TUBES, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | sodium spermine | ||
| 4 | 1 | 2 | CaCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | reservoir | sodium MES | 50 (mM) | pH6.5 |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | beta-octyl glucopyranoside | 0.05 (%) | |
| 5 | 1 | reservoir | sodium spermine | 0.05 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 7 | 1 | reservoir | PEG3350 | 10 (%) |
