1LE8
Crystal Structure of the MATa1/MATalpha2-3A Heterodimer Bound to DNA Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 1999-05-05 |
| Detector | KODAK |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 54.170, 54.170, 164.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.280 - 2.300 |
| R-factor | 0.255 * |
| Rwork | 0.256 |
| R-free | 0.29800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.180 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.030 * | 0.294 * |
| Total number of observations | 48164 * | |
| Number of reflections | 11201 | |
| <I/σ(I)> | 19.6 | 3 |
| Completeness [%] | 92.5 | 53.2 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% PEG400, 8 mM Co(NH3)6Cl3, 10 mM CaCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG400 | ||
| 2 | 1 | 1 | Co(NH3)6Cl3 | ||
| 3 | 1 | 1 | CaCl2 |
