1LDT
COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.400, 63.400, 131.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PORCINE TRYPSIN MODEL FROM TRYPSIN:MUNG BEAN INHIBITOR (LIN ET AL. EUR. J. BIOCHEM. 212 549-555 (1993) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 24.600 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.082 | 0.200 |
| Total number of observations | 139563 * | |
| Number of reflections | 21466 | |
| <I/σ(I)> | 9 | 4 |
| Completeness [%] | 98.6 | 87.6 |
| Redundancy | 6.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 10% PEG 6000, 2.3M PHOSPHATE, PH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Mops | 20 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | reservoir | PEG6000 | 10 (%) | |
| 5 | 1 | reservoir | phosphate | 2.3 (M) |
