1LDJ

Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF Ubiquitin Ligase Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	170
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.943
Spacegroup name	P 1 21 1
Unit cell lengths	113.974, 49.968, 135.883
Unit cell angles	90.00, 107.83, 90.00

Refinement procedure

Resolution	15.000 - 3.000
R-factor	0.247 *
Rwork	0.247
R-free	0.28800
Structure solution method	MIR
RMSD bond length	0.008 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000
High resolution limit [Å]	3.000
Rmerge	0.065 *	0.404 *
Total number of observations	92525 *
Number of reflections	28766
Completeness [%]	97.6 *	97 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	10% Ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	10% Ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	100 (mM)
2	1	reservoir	ethanol	12-18 (%)
3	1	reservoir		200-500 (mM)
4	1	reservoir	dithiothreitol	5 (mM)	pH8.0
5	1	drop	protein	10-20 (mg/ml)