1LD9

THE THREE-DIMENSIONAL STRUCTURE OF AN H-2LD PEPTIDE COMPLEX EXPLAINS THE UNIQUE INTERACTION OF LD WITH BETA2M AND PEPTIDE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	1995-08-27
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	150.100, 87.200, 80.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.400
R-factor	0.186
Rwork	0.186
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hoc
RMSD bond length	0.014 *
RMSD bond angle	2.100 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	0.130
Number of reflections	28578
<I/σ(I)>	3.7	3.7
Completeness [%]	67.0	58
Redundancy	2.4	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	5 (mg/ml)
2	1	drop		0.7 (M)
3	1	drop	glycerol	1-4 (%)
4	1	reservoir		1.4 (M)
5	1	reservoir	glycerol	2-8 (%)