1LCN
Monoclinic hen egg white lysozyme, thiocyanate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.010, 63.180, 60.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.830 - 1.630 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MONOMER A FROM 5LYM PDB ENTRY (MONOCLINIC LYSOZYME) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.350 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.830 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.028 * | |
| Number of reflections | 25539 | |
| <I/σ(I)> | 15.2 | 6.3 |
| Completeness [%] | 96.8 | 89.5 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 * | 291 * | KSCN 190MM BUFFER CH3COOK 50MM, PH=5, pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.7 (mg/ml) | |
| 2 | 1 | drop | potassium acetate | 50 (mM) | |
| 3 | 1 | drop | 190 (mM) |
