1LCL
CHARCOT-LEYDEN CRYSTAL PROTEIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1994 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8, 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 49.600, 49.600, 262.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 1.800 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.25800 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.610 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.077 |
Total number of observations | 75363 * |
Number of reflections | 18046 |
Completeness [%] | 94.4 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 10.5 * | 16 * | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | Tris | 0.1 (M) | |
3 | 1 | reservoir | Tris-acetate | 0.2 (M) |