1LCJ
SH2 (SRC HOMOLOGY-2) DOMAIN OF HUMAN P56-LCK TYROSINE KINASE COMPLEXED WITH THE 11 RESIDUE PHOSPHOTYROSYL PEPTIDE EPQPYEEIPIYL
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.190, 57.310, 31.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.800 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.485 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 1.800 * | 1.800 * |
| Rmerge | 0.084 * | |
| Total number of observations | 47097 * | |
| Number of reflections | 9956 * | |
| Completeness [%] | 85 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 | 25-35 (%) | can be replaced by PEG4000 or PEG8000 |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
