1LCI
FIREFLY LUCIFERASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-10 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.530, 119.530, 94.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26500 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.186 |
| Number of reflections | 45322 | |
| <I/σ(I)> | 11.7 | 4 |
| Completeness [%] | 96.5 | 97.3 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.8 | 4 * | or 10 degrees centigrade * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 10 | 1 | 2 | Tris-HCl | 100 (mM) | |
| 2 | 1 | 1 | ammonium sulfate | 200 (mM) | |
| 3 | 1 | 1 | EDTA | 1 (mM) | |
| 4 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 5 | 1 | 1 | glycerol | 10 (%(v/v)) | |
| 6 | 1 | 1 | ehthylene glycol | 125 (%(v/v)) | |
| 7 | 1 | 1 | Tris-HCl | 25 (mM) | |
| 8 | 1 | 2 | lithium sulphate | 500-540 (mM) | |
| 9 | 1 | 2 | PEG8000 | 26 (%(w/v)) |
