1LC4
Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.900, 32.800, 52.000 |
| Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.540 |
| Rwork | 0.214 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j7t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.640 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.059 * | 0.133 * |
| Number of reflections | 4899 * | |
| <I/σ(I)> | 19.5 | 9.2 |
| Completeness [%] | 95.9 | 98.4 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 310 | MPD, sodium cacodylate, sodium chloride, potassium chloride, magnesium sulfate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 1 | MgSO4 | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0.75-2 (%) | ||
| 2 | 1 | drop | glycerol | 0.5-1 (%) | |
| 3 | 1 | drop | tobramycin | 2.2-2.3 (mM) | |
| 4 | 1 | drop | 56.25-131.25 (mM) | or KCl | |
| 5 | 1 | drop | sodium cacodylate | 62.5 (mM) | pH6.4 |
| 6 | 1 | drop | RNA | 0.5-1.55 (mM) | |
| 7 | 1 | drop | 2.5 (mM) | ||
| 8 | 1 | reservoir | MPD | 40 (%) |
