1LBT

LIPASE (E.C.3.1.1.3) (TRIACYLGLYCEROL HYDROLASE)

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1990-06-18
Detector	XUONG-HAMLIN MULTIWIRE MARK III
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	95.100, 50.200, 99.500
Unit cell angles	90.00, 90.60, 90.00

Refinement procedure

Resolution	7.500 - 2.500
R-factor	0.172
Rwork	0.172
R-free	0.19300
RMSD bond length	0.007
RMSD bond angle	23.800 *
Data reduction software	MARK (III)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500	2.500 *
Rmerge	0.041	0.123 *
Total number of observations	45529 *
Number of reflections	26677
Completeness [%]	82.0 *	63 *
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20-25 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.0 (M)
2	1	reservoir	dioxane	10 (%)
3	1	reservoir	sodium citrate	0.1 (M)
4	1	drop	protein	10 (mg/ml)
5	1	drop	beta-octylglucoside	4 (%(w/v))
6	1	drop	ammonium sulfate	1.0 (1.0)
7	1	drop	dioxane	10 (%)
8	1	drop	sodium citrate	0.1 (M)