1LBM
CRYSTAL STRUCTURE OF PHOSPHORIBOSYL ANTHRANILATE ISOMERASE (PRAI) IN COMPLEX WITH REDUCED 1-(O-CARBOXYPHENYLAMINO)-1-DEOXYRIBULOSE 5-PHOSPHATE (RCDRP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-20 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1995-07-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 68.835, 68.834, 187.163 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
Rwork | 0.170 |
R-free | 0.22800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1nsj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.800 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.800 |
Rmerge | 0.099 |
Total number of observations | 28569 * |
Number of reflections | 6978 |
Completeness [%] | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 277 | 50 mM potassium phosphate , 2.1 M ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 19 (mg/ml) | |
2 | 1 | reservoir | MES | 0.1 (M) | pH7.0 |
3 | 1 | reservoir | ammonium sulfate | 1.5 (M) |