1LBE
APLYSIA ADP RIBOSYL CYCLASE
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1995 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.200, 72.300, 125.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.29900 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.400 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.400 * |
Rmerge | 0.103 |
Total number of observations | 84127 * |
Number of reflections | 25029 * |
Completeness [%] | 98.3 |
Redundancy | 3.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 * | 18 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.0 (OD280) | |
2 | 1 | drop | 40 (mM) | ||
3 | 1 | drop | HEPES | 10 (mM) | |
4 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
5 | 1 | reservoir | imidazole-malate | 0.2 (M) |