1LBA

THE STRUCTURE OF BACTERIOPHAGE T7 LYSOZYME, A ZINC AMIDASE AND AN INHIBITOR OF T7 RNA POLYMERASE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	46.500, 62.500, 110.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.19
Rwork	0.190
RMSD bond length	0.017
RMSD bond angle	3.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.086 *
Total number of observations	42968 *
Number of reflections	7142 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
10	1	2	ammonium sulphate	50 (mM)
11	1	2	sodium citrate	50 (mM)
2	1	1	HEPES	20 (mM)
3	1	1		250 (mM)
4	1	1		0.1 (mM)
5	1	1	2-mercaptoethanol	5 (mM)
6	1	1	PEG4000	20 (%(w/v))
7	1	1	ammonium sulphate	50 (mM)
8	1	1	sodium citrate	50 (mM)
9	1	2	PEG4000	20 (%(w/v))