1LB3
Structure of recombinant mouse L chain ferritin at 1.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 150 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.966 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.590, 180.590, 180.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.000 - 1.210 |
| R-factor | 0.1335 |
| Rwork | 0.132 |
| R-free | 0.16000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h96 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.690 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 1.250 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.078 * | 0.602 * |
| Total number of observations | 399787 * | |
| Number of reflections | 74509 | 5144 * |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 5.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 * | Granier, T., (2001) Acta Crystallogr., D57, 1491. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.4 |
| 3 | 1 | reservoir | ammonium sulfate | 0.92 (M) | |
| 4 | 1 | reservoir | 0.4 (%) | ||
| 5 | 1 | reservoir | 3 (mM) |
