1LAJ

The Structure of Tomato Aspermy Virus by X-Ray Crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-04-01
Detector	MARRESEARCH
Wavelength(s)	1.15000
Spacegroup name	I 2 2 2
Unit cell lengths	294.296, 327.393, 382.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.400
Rwork	0.218
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f15
RMSD bond length	0.009
RMSD bond angle	29.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.720	3.460
High resolution limit [Å]	3.400	3.400
Rmerge	0.230
Total number of observations	1444765 *
Number of reflections	156468
<I/σ(I)>	7.9
Completeness [%]	62.0 *	2.2
Redundancy	1.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	18 *	14-17% Ethanol, 100mM tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	8.5	18 *	14-17% Ethanol, 100mM tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)	pH8.5
2	1	drop	ethanol	14-17 (%)
3	1	drop	Tris	100 (mM)
4	1	reservoir	Tris	100 (mM)	pH8.5
5	1	reservoir	MPD	20 (%)
6	1	reservoir	ethanol	10 (%)
7	1	reservoir	sodium azide	0.04 (%)