1L9Z

Thermus aquaticus RNA Polymerase Holoenzyme/Fork-Junction Promoter DNA Complex at 6.5 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.94799
Spacegroup name	P 43 2 2
Unit cell lengths	180.947, 180.947, 523.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.000 - 6.500
R-factor	0.448 *
R-free	0.45200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	6.730
High resolution limit [Å]	6.500	6.500
Rmerge	0.085	0.241 *
Number of reflections	16195
<I/σ(I)>	11.5
Completeness [%]	90.9	78.5
Redundancy	5.7	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	4 *	ammonium sulfate, Tris, Magnesium chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	ammonium sulfate
2	1	1	Tris
3	1	1	MgCl2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	1.6-1.8 (M)
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.0
4	1	reservoir		20 (mM)