1L9B

X-Ray Structure of the Cytochrome-c(2)-Photosynthetic Reaction Center Electron Transfer Complex from Rhodobacter sphaeroides in Type II Co-Crystals

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-18
Detector	ADSC QUANTUM 4
Wavelength(s)	1.10
Spacegroup name	P 1 21 1
Unit cell lengths	78.222, 115.650, 79.677
Unit cell angles	90.00, 110.29, 90.00

Refinement procedure

Resolution	50.000 * - 2.400
R-factor	0.22 *
Rwork	0.220
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID Code 1AIJ and 1CXC are starting models for the reaction center and cytochrome c2 respectively.
RMSD bond length	0.008
RMSD bond angle	0.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.730	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.100 *	0.326 *
Total number of observations	196158 *
Number of reflections	50331
<I/σ(I)>	4.6	2.1
Completeness [%]	96.8	83.7
Redundancy	3.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	19 *	10% PEG 4000, 0.06%(w/v) lauryldimethylamine-N-oxide, 3.9%(w/v) heptane-1,2,3-triol, 15 mM Tricine, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.7 (mg/ml)
2	1	drop	cytochrome c2	2 (mg/ml)
3	1	drop	PEG4000	10 (%(w/v))
4	1	drop	LDAO	0.06 (%(w/v))
5	1	drop	heptanetriol	3.9 (%(w/v))
6	1	drop	Tricine	15 (mM)	pH8.5
7	1	reservoir	PEG4000	22 (%(w/v))
8	1	reservoir	Tricine	50 (mM)	pH8.5