1L9A
CRYSTAL STRUCTURE OF SRP19 IN COMPLEX WITH THE S DOMAIN OF SIGNAL RECOGNITION PARTICLE RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9185 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 70.765, 223.875, 43.024 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.840 - 2.900 |
| R-factor | 0.264 |
| Rwork | 0.264 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 17.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 0.310 |
| Number of reflections | 26760 | |
| <I/σ(I)> | 18.7 | 3.1 |
| Completeness [%] | 91.4 | 52.9 |
| Redundancy | 6.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 27.5 * | 12% PEG4000, 110MM Tris.HCl pH 7.4, 10% glycerol, 50mm magnesium acetate, 100MM ammonium acetate, 5MM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 300.5K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | ammonium acetate | 100 (mM) | |
| 3 | 1 | drop | Tris-HCl | 10 (mM) | pH7.4 |
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | dithiothreitol | 5 (mM) | |
| 7 | 1 | reservoir | PEG4000 | 12 (%(w/v)) | |
| 8 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.4 |
| 9 | 1 | reservoir | magnesium acetate | 50 (mM) |
