1L8V
Crystal Structure of a Mutant (C109G,G212C) P4-P6 Domain of the Group I Intron from Tetrahymena Thermophilia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.455, 129.918, 144.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 * - 2.800 |
| R-factor | 0.29 |
| Rwork | 0.290 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gid |
| RMSD bond length | 0.006 |
| RMSD bond angle | 15.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.980 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.051 * | 0.160 * |
| Number of reflections | 33255 | |
| <I/σ(I)> | 32.9 | |
| Completeness [%] | 87.3 | 95.2 * |
| Redundancy | 7.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 * | 25 * | MPD, magnesium chloride, cacodylate, spermine, sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | MgCl | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 1 | spermine | ||
| 5 | 1 | 1 | NaCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 22 (%) | ||
| 2 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.5 |
| 3 | 1 | reservoir | spermine | 0.05 (mM) | |
| 4 | 1 | drop | protein | 4 (mg/ml) | |
| 5 | 1 | drop | HEPES-NaOH | 5 (mM) | pH7.5 |
| 6 | 1 | drop | 5 (mM) | ||
| 7 | 1 | drop | 40-70 (mM) |
