1L8R
Structure of the Retinal Determination Protein Dachshund Reveals a DNA-Binding Motif
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97981, 0.97904, 0.95366 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.797, 47.731, 50.436 |
| Unit cell angles | 60.75, 86.86, 84.55 |
Refinement procedure
| Resolution | 6.000 * - 1.650 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.25100 * |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.120 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.064 * | 0.233 * |
| Total number of observations | 227661 * | |
| Number of reflections | 26123 * | |
| Completeness [%] | 96.5 | 91.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 277 | PEG 10000, Ammonium Sulphate, pH 7.5, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG10000 | 25-30 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.25 (M) | |
| 3 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | protein | 8 (mg/ml) |
