1L8N
The 1.5A crystal structure of alpha-D-glucuronidase from Bacillus stearothermophilus T-1, complexed with 4-O-methyl-glucuronic acid and xylotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2001-07-12 |
| Detector | BRANDEIS |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.946, 73.946, 331.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| Rwork | 0.140 |
| R-free | 0.17210 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | pdb code 1k9d |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.130 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.110 | 0.399 |
| Number of reflections | 145976 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 98.8 | 90.3 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M NA-CITRATE PH 5.0, 14% PEG 4000, 12% ISO-PROPANOL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
