1L8F

Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1998-09-24
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	47.440, 47.440, 156.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 * - 1.800
Rwork	0.199
R-free	0.24700 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.350 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.091	0.251 *
Total number of observations	78044 *
Number of reflections	16780 *
Completeness [%]	95.6	88.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2 *	298	peg 8000, Na-acetate, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop	Tris-HCl	12.5 (mM)	pH7.2
3	1	drop	EDTA	12.5 (mM)
4	1	drop	sodium azide	0.01 (%)
5	1	reservoir	sodium acetate	100 (mM)	pH5.10
6	1	reservoir	PEG8000	5 (%)