1L8D
Rad50 coiled-coil Zn hook
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8321, 1.009 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.958, 77.868, 53.330 |
| Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| Rwork | 0.225 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.790 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.076 * | 0.344 * |
| Total number of observations | 133282 * | |
| Number of reflections | 13119 | |
| Completeness [%] | 88.0 | 62.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 300 | 100 mM Phosphate/Citrate, 40% Ethanol, 50 mM Zn-Acetate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 10 | 1 | reservoir | zinc acetate | 20 (mM) | |
| 2 | 1 | drop | phosphate | 20 (mM) | pH7.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | glycerol | 5 (%) | |
| 6 | 1 | drop | dithiothreitol | 2 (mM) | |
| 7 | 1 | reservoir | phosphate/citrate | 100 (mM) | pH4.2 |
| 8 | 1 | reservoir | ethanol | 40 (%) | |
| 9 | 1 | reservoir | glycerol | 10 (%) |
