1L8B

Cocrystal Structure of the Messenger RNA 5' Cap-binding Protein (eIF4E) bound to 7-methylGpppG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-12-04
Detector	PRINCETON 2K
Wavelength(s)	0.91
Spacegroup name	P 21 21 21
Unit cell lengths	70.120, 75.920, 77.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.224 *
Rwork	0.224
R-free	0.25200
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.820
High resolution limit [Å]	1.800	1.800
Rmerge	0.103 *
Number of reflections	38968
Completeness [%]	97.8	87.5
Redundancy	7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6	277	Marcotrigiano, J., (1997) Cell, 89, 951. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES	100 (mM)
2	1	reservoir	PEG4000	10-12 (%)
3	1	reservoir	isopropanol	10 (%)
4	1	reservoir	dithiothreitol	20 (mM)