1L7T

Crystal Structure Analysis of the anti-testosterone Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1999-09-23
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	71.695, 88.419, 143.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 * - 2.100
Rwork	0.213
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.340 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000 *	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.326 *
Total number of observations	120393 *
Number of reflections	25386 *
Completeness [%]	93.6	87.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6 *	298	Valjakka, J., (2002) J.Biol.Chem., 277, 4183. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop	Bis-Tris propane	0.1 (M)	pH5.6
3	1	drop	PEG3350	15 (%)