1L5B

DOMAIN-SWAPPED CYANOVIRIN-N DIMER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	61.966, 61.966, 148.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.070 *
R-factor	0.237 *
Rwork	0.247
R-free	0.24500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ezm
RMSD bond length	0.029 *
RMSD bond angle	27.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.130
High resolution limit [Å]	2.070 *	2.000
Rmerge	0.049
Total number of observations	392421 *
Number of reflections	20437 *
Completeness [%]	99.4 *	99.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.3	295	sodium citrate, CHES, pH 10.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K