1L3Z
Crystal Structure Analysis of an RNA Heptamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 25.740, 106.580, 30.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.010 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.18500 * |
| RMSD bond length | 0.003 |
| RMSD bond angle | 10.500 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.000 * | 2.010 |
| Rmerge | 0.054 * | |
| Number of reflections | 2840 * | |
| Completeness [%] | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | MPD, CaCl2, Cacodylate buffer, pH7.0 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | Cacodylate buffer |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 2 (mM) | single strand concentration |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | pH7.0 |
| 3 | 1 | drop | 24 (mM) | ||
| 4 | 1 | drop | MPD | 2 (%(v/v)) | |
| 5 | 1 | reservoir | MPD | 40 (%) |
