1L3D
Low Resolution Crystal Structure of a Viral RNA Pseudoknot
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0800 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 107.810, 107.810, 107.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.850 |
| R-factor | 0.271 * |
| Rwork | 0.271 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 437d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.054 | 0.286 |
| Number of reflections | 5034 | |
| <I/σ(I)> | 39.5 | 9.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 4 * | ammonium sulfate, sodium citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | sodium citrate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 2.7 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1 (M) | |
| 3 | 1 | drop | sodium citrate | 33 (mM) | pH5.0 |
| 4 | 1 | reservoir | ammonium sulfate | 2 (M) |
