1L2O

SCALLOP MYOSIN S1-ADP-p-PDM IN THE ACTIN-DETACHED CONFORMATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-03-26
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 1
Unit cell lengths	51.800, 57.000, 150.500
Unit cell angles	95.60, 96.30, 101.50

Refinement procedure

Resolution	54.160 - 2.800
R-factor	0.28
Rwork	0.280
R-free	0.32700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kk8
RMSD bond length	0.010
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.980
High resolution limit [Å]	2.800	2.800
Number of reflections	29564
<I/σ(I)>	16
Completeness [%]	74.4	24.4
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	PEG 20000, magnesium chloride, ethylene glycol, Tris HCl, ATPgammaS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K