1L2L

Crystal structure of ADP-dependent glucokinase from a Pyrococcus Horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	280
Detector technology	CCD
Collection date	2001-04-30
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	64.760, 74.730, 99.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.930 - 2.000
R-factor	0.213
Rwork	0.213
R-free	0.28000 *
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	21.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.060
High resolution limit [Å]	2.000	2.000
Rmerge	0.090 *	0.418 *
Number of reflections	275056 *
Completeness [%]	99.1 *	97.1 *
Redundancy	8.3 *	3.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.6	20 *	PEG6000, LiSO4, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG6000	9-13 (%)
3	1	reservoir		0.2 (M)
4	1	reservoir	citrate	0.1 (M)	pH3.6