1L1F
Structure of human glutamate dehydrogenase-apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 2001-02-14 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 97.800, 98.800, 124.200 |
Unit cell angles | 86.26, 70.28, 60.34 |
Refinement procedure
Resolution | 8.000 - 2.700 |
R-factor | 0.262 * |
Rwork | 0.262 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.700 | 2.700 * |
Rmerge | 0.047 * | 0.216 * |
Number of reflections | 94574 * | 7168 * |
Completeness [%] | 90.5 | 54.8 * |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 8000, NaCl, sodium phosphate, sodium azide, octyl-b-glucopyranoside, methyl pentanediol, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | octyl-beta-glucopyranoside | 1 (%(w/v)) | |
2 | 1 | reservoir | sodium azide | 1 (mM) | |
3 | 1 | reservoir | 50 (mM) | ||
4 | 1 | reservoir | methyl pentanediol | 8 (%(v/v)) | |
5 | 1 | reservoir | sodium phosphate | 0.1 (M) | pH7.3 |
6 | 1 | drop | enzyme | 18 (mg/ml) | |
7 | 1 | drop | sodium phosphate | 0.1 (M) | pH7.0 |