1L0W
Aspartyl-tRNA synthetase-1 from space-grown crystals
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1996-11-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.91 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.020, 156.100, 177.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.980 - 2.010 |
R-factor | 0.217 * |
Rwork | 0.217 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 23.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 12.000 * | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.057 | 0.235 |
Total number of observations | 320200 * | |
Number of reflections | 100854 | |
<I/σ(I)> | 17.3 | 2.16 |
Completeness [%] | 93.3 * | 95.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 298 | Ammonium sulfate, Tris-HCl, EDTA, DTT, PMSF, pH 7.5, Under microgravity, temperature 298K |
1 | Vapor diffusion * | 7.5 | 298 | Ammonium sulfate, Tris-HCl, EDTA, DTT, PMSF, pH 7.5, Under microgravity, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 2.2 (M) | |
3 | 1 | drop | Tris-HCl | 25 (mM) | pH7.5 |
4 | 1 | drop | EDTA | 10 (mM) | |
5 | 1 | drop | dithiothreitol | 1 (mM) | |
6 | 1 | drop | PMSF | 0.01 (%(m/v)) |