1L0Q
Tandem YVTN beta-propeller and PKD domains from an archaeal surface layer protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 1999-11-24 |
| Detector | SBC-2 |
| Wavelength(s) | 0.979067,0.979221,0.939270 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 188.181, 65.885, 140.030 |
| Unit cell angles | 90.00, 124.25, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.26300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 28.428 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.109 | 0.434 |
| Total number of observations | 318719 * | |
| Number of reflections | 52881 | |
| <I/σ(I)> | 14.6 | 3.1 |
| Completeness [%] | 93.7 | 89 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 298 | PEG-8000, potassium chloride, sodium acetate, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 20 (%) | |
| 3 | 1 | reservoir | 0.3 (M) | ||
| 4 | 1 | reservoir | 0.1 (M) | pH3.8 |
