1L0P

CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX BETWEEN PSYCHROPHILIC ALPHA AMYLASE FROM PSEUDOALTEROMONAS HALOPLANCTIS AND NITRATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1998-12-28
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	71.300, 138.400, 114.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.560 - 2.100
R-factor	0.156
Rwork	0.146
R-free	0.18010
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1aqh
RMSD bond length	0.006
RMSD bond angle	1.270
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.600	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.150
Total number of observations	149687 *
Number of reflections	29641
Completeness [%]	89.3	90.5
Redundancy	5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	Aghajari, N., (1996) Protein Sci., 5, 2128. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MPD	60-70 (%)
2	1	reservoir	HEPES	0.1 (M)