1L0B

Crystal Structure of rat Brca1 tandem-BRCT region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	103
Detector technology	CCD
Collection date	2001-05-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.928
Spacegroup name	C 1 2 1
Unit cell lengths	84.512, 58.722, 65.327
Unit cell angles	90.00, 111.28, 90.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.203 *
R-free	0.25800 *
RMSD bond length	0.011 *
RMSD bond angle	1.610 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.082 *	0.457 *
Total number of observations	55198 *
Number of reflections	17223
Completeness [%]	97.0 *	97.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	PEG 8000, Tris-hydrochloride, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	PEG 8000, Tris-hydrochloride, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	PEG 8000, Tris-hydrochloride, sodium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	50 (mM)	pH8.5
2	1	reservoir		150 (mM)
3	1	reservoir	PEG8000	18 (%(w/v))
4	1	reservoir	dithiothreitol	5 (mM)
5	1	drop	protein	75 (mg/ml)
6	1	drop	sodium HEPES	10 (mM)	pH7.5
7	1	drop		150 (mM)
8	1	drop	dithiothreitol	5 (mM)