1KZP
PROTEIN FARNESYLTRANSFERASE COMPLEXED WITH A FARNESYLATED K-RAS4B PEPTIDE PRODUCT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 171.239, 171.239, 69.361 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d8d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.086 * | 0.569 * |
| Total number of observations | 402441 * | |
| Number of reflections | 67840 | |
| <I/σ(I)> | 21 | 2.47 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.93 | 5.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 17 * | Park, H.W., (1997) Science, 275, 1800. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | FTase | 16 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | Tris-HCl | 20 (mM) | |
| 6 | 1 | drop | PEG8000 | 13-15 (%(w/v)) | |
| 7 | 1 | drop | ammonium acetate | 200 (mM) | |
| 8 | 1 | reservoir | PEG8000 | 13-15 (%(w/v)) | |
| 9 | 1 | reservoir | ammonium acetate | 200 (mM) |
