1KZO

PROTEIN FARNESYLTRANSFERASE COMPLEXED WITH FARNESYLATED K-RAS4B PEPTIDE PRODUCT AND FARNESYL DIPHOSPHATE SUBSTRATE BOUND SIMULTANEOUSLY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-07
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	171.199, 171.199, 69.444
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.159
Rwork	0.159
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d8d
RMSD bond length	0.007
RMSD bond angle	20.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.200	2.200
Rmerge	0.089 *	0.560 *
Total number of observations	253335 *
Number of reflections	56209
<I/σ(I)>	16.1	1.8
Completeness [%]	96.0	87.9
Redundancy	4.51	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	17 *	Long, S.B., (2000) Structure, 8, 209. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	12-16 (%(w/v))
2	1	reservoir	ammonium acetate	600 (mM)
3	1	reservoir	dithiothreitol	20 (mM)