1KYX

Lumazine Synthase from S.pombe bound to carboxyethyllumazine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	111.087, 145.178, 128.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.460 * - 2.600
R-factor	0.178
Rwork	0.174
R-free	0.20600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.440 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.467	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.070 *	0.420 *
Number of reflections	32116
Completeness [%]	99.5 *	99.5 *
Redundancy	3.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7 *	295	citrate, ammonium dihydrogen phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	10 (mg/ml)
2	1	drop	potassium phosphate	20 (mM)	pH7.0
3	1	drop		50 (mM)
4	1	reservoir	citrate	0.1 (M)	pH4.9-5.2
5	1	reservoir	sodium formate	1.5 (M)