1KYN
Cathepsin-G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 59.440, 59.440, 130.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.860 - 3.500 |
| Rwork | 0.258 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.100 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.090 | 0.200 |
| Number of reflections | 7354 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 79.7 | 75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
