1KYH
Structure of Bacillus subtilis YxkO, a Member of the UPF0031 Family and a Putative Kinase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-25 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795,0.9798,0.94656 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 91.900, 91.900, 170.506 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.600 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.146 | 0.546 |
| Number of reflections | 48555 * | 4216 * |
| <I/σ(I)> | 31 | 2.09 |
| Completeness [%] | 97.3 | 86 |
| Redundancy | 17 | 4.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.15 M MgCl2, 28% PEG400, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | 28 (%) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | reservoir | 0.15 |
