1KYF
AP-2 CLATHRIN ADAPTOR ALPHA-APPENDAGE IN COMPLEX WITH EPS15 DPF PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.620, 72.820, 41.860 |
| Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.220 |
| R-factor | 0.153 * |
| Rwork | 0.153 |
| R-free | 0.21100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qts |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.260 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.059 * | 0.388 * |
| Total number of observations | 402957 * | |
| Number of reflections | 67213 | |
| <I/σ(I)> | 15.6 | 2.3 |
| Completeness [%] | 94.5 | 84 |
| Redundancy | 6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein/peptide solution | 6 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.36-1.49 (M) | |
| 3 | 1 | reservoir | HEPES | 85-93 (mM) | pH6.8 |
| 4 | 1 | reservoir | dioxane | 8.5-9.3 (%) | |
| 5 | 1 | reservoir | glycerol | 0-5 (%) |
