1KXV

Camelid VHH Domains in Complex with Porcine Pancreatic alpha-Amylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	57.568, 60.990, 107.333
Unit cell angles	98.78, 100.88, 101.14

Refinement procedure

Resolution	30.000 * - 1.700 *
R-factor	0.205 *
Rwork	0.205
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fjh PDB ENTRY 1QD0
RMSD bond length	0.020
RMSD bond angle	25.800 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.690
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.074	0.137
Total number of observations	716803 *
Number of reflections	175069
<I/σ(I)>	4.6	2
Completeness [%]	99.8 *	99.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	293	PEG4000 32%, Na citrate 0.1M & Ammonium Acetate 0.2M, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	50 (mM)	pH7.5
2	1	drop		100 (mM)
3	1	reservoir	PEG4000	32 (%)
4	1	reservoir	sodium citrate	0.1 (M)
5	1	reservoir	ammonium acetate	0.2 (M)	pH5.0