1KXK
Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 90.987, 90.987, 235.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.2497 |
| Rwork | 0.248 |
| R-free | 0.26800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.158 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.054 * | 0.430 * |
| Number of reflections | 10014 | |
| <I/σ(I)> | 23.4 | 3 |
| Completeness [%] | 85.8 * | 51.7 |
| Redundancy | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 25 * | HEPES-KOH, magnesium chloride, ammonium sulfate, spermine, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 25 * | HEPES-KOH, magnesium chloride, ammonium sulfate, spermine, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES-KOH | ||
| 2 | 1 | 1 | MgCl | ||
| 3 | 1 | 1 | ammonium sulfate | ||
| 4 | 1 | 1 | spermine |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 5-6 (mg/ml) | |
| 2 | 1 | drop | 2.5 (mM) | ||
| 3 | 1 | drop | HEPES-KOH | 10 (mM) | pH7.0 |
| 4 | 1 | reservoir | HEPES-KOH | 50 (mM) | pH7.0 |
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | ammonium sulfate | 1-1.2 (M) | |
| 7 | 1 | reservoir | spermine | 0.1 (mM) |
