1KX9

ANTENNAL CHEMOSENSORY PROTEIN A6 FROM THE MOTH MAMESTRA BRASSICAE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9789
Spacegroup name	P 1 21 1
Unit cell lengths	47.600, 49.700, 50.300
Unit cell angles	90.00, 110.10, 90.00

Refinement procedure

Resolution	15.000 * - 1.650
Rwork	0.204
R-free	0.20400 *
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	19.100 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	1.750
High resolution limit [Å]	1.600 *	1.600 *
Rmerge	0.038	0.245
Number of reflections	25956
<I/σ(I)>	11.8	7.2
Completeness [%]	93.3 *	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	293	Campanacci, V., (2001) Acta Crystallogr., Sect.D, 57, 137. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	46 (mg/ml)
2	1	drop	Tris	50 (mM)	pH8.0
3	1	reservoir	PEG5000 MME	36 (%)
4	1	reservoir	sodium acetate	0.2 (M)	pH5.5