1KX8

Antennal Chemosensory Protein A6 from Mamestra brassicae, tetragonal form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-05
Detector	MARRESEARCH
Wavelength(s)	0.97888, 0.97909, 0.91839
Spacegroup name	P 43 21 2
Unit cell lengths	69.406, 69.406, 79.645
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.800
Rwork	0.265
R-free	0.26500 *
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	18.700 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.110	0.320
Number of reflections	5096
<I/σ(I)>	22	4.5
Completeness [%]	99.0 *	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	293	Campanacci, V., (2001) Acta Crystallogr., Sect.D, 57, 137. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	46 (mg/ml)
2	1	drop	Tris	50 (mM)	pH8.0
3	1	reservoir	PEG5000 MME	36 (%)
4	1	reservoir	sodium acetate	0.2 (M)	pH5.5