1KX3
X-Ray Structure of the Nucleosome Core Particle, NCP146, at 2.0 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID09 |
| Synchrotron site | ESRF |
| Beamline | ID09 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.85 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.400, 181.540, 109.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.241 |
| Rwork | 0.240 |
| R-free | 0.27500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1aoi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.040 |
| High resolution limit [Å] | 2.000 * | 2.000 * |
| Rmerge | 0.068 | 0.495 |
| Number of reflections | 145317 | |
| Completeness [%] | 98.3 | 84.5 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 293 | manganese chloride, potassium chloride, potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MnCl2 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | potassium cacodylate |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | 70-85 (mM) | ||
| 3 | 1 | drop | 50-60 (mM) | ||
| 4 | 1 | drop | potassium cacodylate | 20 (mM) | pH6.0 |
| 5 | 1 | reservoir | 40-46 (mM) | ||
| 6 | 1 | reservoir | 30-40 (mM) | ||
| 7 | 1 | reservoir | potassium cacodylate | 10-20 (mM) |
