1KX1
Rat mannose protein A complexed with Man6-GlcNAc2-Asn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12-08 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.800, 255.560, 61.790 |
| Unit cell angles | 90.00, 119.60, 90.00 |
Refinement procedure
| Resolution | 37.040 - 2.800 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 37844 | |
| Completeness [%] | 94.4 | 75.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | 10% PEG 8000, 100mM Tris-Cl pH=8.0, 10mM NaCl, 20mM Cacl2, 2mM NaN3. Protein solution: 5mg/ml in 10mM Tris-Cl pH=8.0, 10 mM NaCl, 20mM CaCl2, 1.1mM Man6GlcNAc2Asn. VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | 2 (mM) | ||
| 2 | 1 | drop | Tris-Cl | 10 (mM) | pH8.0 |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | Man6GlcNAc2Asn | 1.1 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
| 7 | 1 | reservoir | Tris-Cl | 100 (mM) | pH8.0 |
| 8 | 1 | reservoir | 10 (mM) | ||
| 9 | 1 | reservoir | 20 (mM) |
