1KWP

Crystal Structure of MAPKAP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	110
Detector technology	CCD
Collection date	2000-12-10
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	H 3
Unit cell lengths	143.944, 143.944, 90.273
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.600 - 2.800
R-factor	0.233
Rwork	0.233
R-free	0.24500
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	3.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNX
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.600	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.066 *	0.302
Total number of observations	386514 *
Number of reflections	17189 *
<I/σ(I)>	4.9	4.9
Completeness [%]	100.0	100
Redundancy	11.3	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.8 *	298	2 M Na/K phosphate, pH 5.15, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	25 (mM)	pH7.8
2	1	drop		200 (mM)
3	1	drop	dithiothreitol	2 (mM)
4	1	drop	protein	5-10 (mg/ml)
5	1	reservoir	sodium potassium phospahte	2 (M)	pH5.15